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protean isoelectric focusing (ief) tray  (Bio-Rad)


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    Structured Review

    Bio-Rad protean isoelectric focusing (ief) tray
    Protean Isoelectric Focusing (Ief) Tray, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isoelectric+focusing+(ief)+tray/Focusing+Tray+With+Lid/pmc06912385-117-10-12
    Average 90 stars, based on 1 article reviews
    protean isoelectric focusing (ief) tray - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Electrofocusing:

    Article Title: Drosophila NUAK functions with Starvin/BAG3 in autophagic protein turnover.
    Article Snippet: .. The samples were transferred to separate lanes of a BioRad Protean IEF focusing tray along with BioRad ReadyStrip IPG strips, pH 5–8, 7cm (BioRad Laboratories, Hercules, CA) and covered with mineral oil. .. Using a BioRad Protean IEF cell, the strips were passively rehydrated (20 ̊C for 12 hrs) and then focused (4000V, Rapid, 15000 Vhrs).

    Article Title: Iron-Induced Changes in the Proteome of Trichomonas vaginalis Hydrogenosomes
    Article Snippet: .. Combined iTRAQ-labeled samples were dissolved in 250 μl of 2 M urea and poured into the 17 cm focusing tray of a Protean IEF Cell (Bio-Rad, Hercules, CA, USA). .. The sample was covered with 17 cm IPG strips (pH 3–10, Bio-Rad) without paper wicks or oil.

    Article Title: BASP1 labels neural stem cells in the neurogenic niches of mammalian brain
    Article Snippet: .. The strips were transferred to a Protean IEF (BIO-RAD) focusing tray and laid gel side down across wet paper wick-covered electrodes and covered with mineral oil. .. The strips were focused in a Bio-Rad Protean IEF Cell at 20 °C, rapid ramp 0–250 V, 15 min; rapid ramp 250–8000 V 2 h; hold 8000 V to a total of 35,000 Vh.

    Article Title: Comparative brain proteome analysis of an Indole Alkaloid of Kratom, Mitragynine and Kratom juice in rats.
    Article Snippet: Mitragyna speciosa, commonly known as kratom, is a medicinal plant that is widely used for various medical conditions.. The major alkaloid in kratom is mitragynine which binds partially to opioid receptors to produce opioid-like effects.. In this study, we compared the effects of mitragynine various doses with kratom juice on neurobehavioral, neurochemical changes as well as calretinin protein expression.

    Article Title: Histological and proteome analyses of Microbacterium foliorum -mediated decrease in arsenic toxicity in Melastoma malabathricum .
    Article Snippet: Subsequently, the electrode tray was covered and put into the PROTEAN IEF Cell (Bio-Rad, USA) and the IEF method was specified. .. The electrode tray was removed from the PROTEAN IEF Cell (Bio-Rad) after completion of the IEF, and the strips were transferred upside down to a strip holder gel. .. SDS equilibration buffer (6 M urea, 75 mM pH 8.8 Tris HCl, 29.3% (v/v) glycerol, 2% (w/v) SDS, 0.02% (w/v) bromophenol blue) with DTT (100 mg/10 mL) was first added, then SDS 3 Biotech (2021) 11:336 1 3 336 Page 4 of 17 equilibration buffer with iodoacetamide (250 mg/10 mL) was used and the strip holder was shaked for 15 min on a gyro-rocker.

    Article Title: Histological and proteome analyses of Microbacterium foliorum -mediated decrease in arsenic toxicity in Melastoma malabathricum .
    Article Snippet: .. Subsequently, the electrode tray was covered and put into the PROTEAN IEF Cell (Bio-Rad, USA) and the IEF method was specified. .. The electrode tray was removed from the PROTEAN IEF Cell (Bio-Rad) after completion of the IEF, and the strips were transferred upside down to a strip holder gel.

    Article Title: The Cell Cycle and Virus Infection
    Article Snippet: .. In the IEF tray (Bio-Rad, UK; see manufacturer′s instructions), place a paper wick at both ends of the channels covering the wire electrodes. ..

    Multiplex sample analysis:

    Article Title: Iron-Induced Changes in the Proteome of Trichomonas vaginalis Hydrogenosomes
    Article Snippet: .. Combined iTRAQ-labeled samples were dissolved in 250 μl of 2 M urea and poured into the 17 cm focusing tray of a Protean IEF Cell (Bio-Rad, Hercules, CA, USA). .. The sample was covered with 17 cm IPG strips (pH 3–10, Bio-Rad) without paper wicks or oil.

    other:

    Article Title: Oligomeric States and Hydrodynamic Properties of Lysyl Oxidase-Like 2
    Article Snippet: The IPG strips were transferred to the running tray of a PROTEAN ® i12TM IEF system (Bio-Rad Laboratories) before being overlaid with another 5 mL of mineral oil and run using the recommended procedure for 11 cm pH 3–10 IPG strips (e.g., 250 volts for 20 min, 8000 volts for 1 h, and 8000 volts for 26,000 volt hours, with a total run time of approximately 6 h at 20 °C).

    Stripping Membranes:

    Article Title: Histological and proteome analyses of Microbacterium foliorum -mediated decrease in arsenic toxicity in Melastoma malabathricum .
    Article Snippet: Subsequently, the electrode tray was covered and put into the PROTEAN IEF Cell (Bio-Rad, USA) and the IEF method was specified. .. The electrode tray was removed from the PROTEAN IEF Cell (Bio-Rad) after completion of the IEF, and the strips were transferred upside down to a strip holder gel. .. SDS equilibration buffer (6 M urea, 75 mM pH 8.8 Tris HCl, 29.3% (v/v) glycerol, 2% (w/v) SDS, 0.02% (w/v) bromophenol blue) with DTT (100 mg/10 mL) was first added, then SDS 3 Biotech (2021) 11:336 1 3 336 Page 4 of 17 equilibration buffer with iodoacetamide (250 mg/10 mL) was used and the strip holder was shaked for 15 min on a gyro-rocker.



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